University of Houston, Texas
Research Assistant, Department of Engineering Technology
Protein L is a gram-positive bacterium derived from Peptostreptococcus magnus, binds to various immunoglobulin antibodies through its multiple binding domains that interact with kappa light chains. This unique capability has sparked a rising interest in the field of biotechnology and medicine, positioning it as a valuable tool for therapeutic antibody production, and also enabling the purification of different types of antibodies from complex biological samples, without interfering with their antigen-binding properties.
I worked as a Research Assistant at the University of Houston during Spring 2024 to carry out my bachelors thesis focused on the expression of recombinant Protein L in E. coli using bioreactor and employing purification strategies to investigate its binding efficacy for enhancing therapeutic applications. Additionally, I purified the protein produced using affinity chromatography techniques like Ni-NTA and IgG Sepharose using an AKTA purifier, along with protein quantification analysis using BCA assay, SDS-PAGE, and MALDI-ToF.
